منابع مشابه
Cell Culture Assay for Human Noroviruses
To the Editor: We read with great interest the article on human norovirus (hNoV) by Straub et al. (1). By using 3-dimensional aggregates of a highly differentiated intestinal epithelial cell line, the investigators claimed to have established an in vitro cell culture model that “support[s] the natural growth of human noroviruses.” While the authors provide compelling evidence of successful viru...
متن کاملIn Vitro Cell Culture Infectivity Assay for Human Noroviruses
Human noroviruses cause severe, self-limiting gastroenteritis that typically lasts 24-48 hours. Because of the lack of suitable tissue culture or animal models, the true nature of norovirus pathogenesis remains unknown. We show, for the first time, that noroviruses can infect and replicate in a physiologically relevant 3-dimensional (3-D), organoid model of human small intestinal epithelium. Th...
متن کاملPorcine Noroviruses Related to Human Noroviruses
Detection of genogroup II (GII) norovirus (NoV) RNA from adult pigs in Japan and Europe and GII NoV antibodies in US swine raises public health concerns about zoonotic transmission of porcine NoVs to humans, although no NoVs have been detected in US swine. To detect porcine NoVs and to investigate their genetic diversity and relatedness to human NoVs, 275 fecal samples from normal US adult swin...
متن کاملEffects of different culture media on optimization of primary neuronal cell culture for in vitro models assay
Background: In vitro model studies are becoming increasingly popular for experimental research designs. They include isolation and expansion of cells of a particular tissue, such as the nervous tissue which contributes to understanding the underlying mechanisms in many pathologies. It enables the scrutinization of intracellular signaling pathways responsible for cell death. OBJECTIVES: In the ...
متن کاملA Cell Culture Assay for Follicle-stimulating
Cultured rat ovarian granulosa cells respond to follicle-stimulating hormone (FSH) by synthesizing and secreting plasminogen activator. The specificity of the response for FSH prompted us to explore the use of this system as an in vitro bioassay for FSH. The release of FSH by pituitary cell cultures has been examined by this method, as have been preparations of FSH of known biological activity....
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ژورنال
عنوان ژورنال: Emerging Infectious Diseases
سال: 2007
ISSN: 1080-6040,1080-6059
DOI: 10.3201/eid1307.070131